Isolation and Characterization of Agrobacterium rhizogenes from the Root Nodules of Some Leguminous Plants

A total of 57 colonies of Agrobacterium species was isolated from the root nodules of five different leguminous plant namely Pisum sativum, Sesbania rostrata, Vigna mungo, V. radita and V. ungiculata by using yeast extract mannitol agar (YEMA). All the isolated colonies were belonged to Agrobacterium species. The isolated bacteria were characterized and identified as Agrobacterium rhizogenes based on the morphological, biochemical, cultural and pathogenicity tests. Biochemical characteristics such as exopolysaccharide, glycogen, total protein, total free amino acids and total lipids were estimated from the agrobacterial isolates. The agrobacteria of different leguminous plants showed lot of variations in their biochemical constituents.